Molecular cloning of cDNAs for Korean garlic viruses

  • Choi, Jin-Nam (Department of Agricultural Chemistry, Seoul National University) ;
  • Ahn, Ji-Hoon (Department of Molecular Biology, Seoul National University) ;
  • Choi, Yang-Do (Department of Agricultural Chemistry, Seoul National University) ;
  • Lee, Jong-Seob (Department of Molecular Biology, Seoul National University)
  • Published : 1993.03.31

Abstract

To understand the molecular structure and pathogenesis mechanism of Korean garlic viruses (GV), virus particles were isolated from field-grown garlic leaves and RNA genome was isolated from them. It was used for constructing cDNA library for GV. Several cDNA clones for GV were isolated and classified into 4 different groups on the basis of cross Southern hybridization. Northern blot analysis of GV RNA with one of these cDNA clones shows that the clone is a cDNA for GV RNA.

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