Abstract
This study was carried out to determine the plasma vitamin $D_3$($D_3$) of 3 weeks old broiler chicks (Hubbard line), whose dorsal skin was exposed to different dose of 310 m UV light. The irradiated doses were 0~0.79 J/$\textrm{cm}^2$(6 doses$\times$5 replica＝30 chicks). The blood samples were collected 2 days after exposure. The lipid in plasma isolated was extracted with ethyl acetate. The $D_3$ was purified by $C_{18}$ cartridge and analyzed by HPLC. The average $D_3$ content in plasma of control chicks was 20 ng /$m\ell$. When 0.32 J/$\textrm{cm}^2$ of dose was treated, the highest amount of 43 ng /$m\ell$ was found among those of chicks experimented, corresponding to 2.15 times that of control. However, When more doses than 0.32 J/$\textrm{cm}^2$ were exposed to the skin, D$_3$ contents were decreased gradually. The similar to control level of $D_3$ was observed when 0.79 J/$\textrm{cm}^2$ was applied.
본 연구는 3주령 브로일러 병아리 (Hubrd 계통)의 등 피부에 310nm자외선 조사시 상이한 선양에 따르는 혈장중 비타민 $D_3$ ( $D_3$ ) 함량을 측정코자 실시되었다. 자외선 조사선량은 0~0.79J/$\textrm{cm}^2$으로 하였으며 (5반복) 2일 후에 채혈하고 혈장을 분리하여 지질을 추출, $C_{18}$ cartridge로 정제, HPLC로 D$_3$를 분석하였다. 무처리구 병아리의 혈장내 $D_3$함량은 20ng/$m\ell$이었으며 0.32J/$\textrm{cm}^2$조사시 43ng/$m\ell$로서 시험한 모든 선양중 가장 높은 값을 보였다. 이 값은 무처리구에 비하여 2.15 배에 해당한다. 그러나 0.48J/$\textrm{cm}^2$ 이상 조사시에는 $D_3$ 함량이 점차 감소되었으며 0.79J/$\textrm{cm}^2$ 처리시에는 무처리구의 수준과 같이 되었다.