Journal of Microbiology and Biotechnology
- 제2권3호
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- Pages.174-182
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- 1992
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Helper-Independent Live Recombinant Adenovirus Vector Expressing the Hemagglutinin-Esterase Membrane Glycoprotein
- YOO, DONGWAN (Veterinary Infectious Disease Organization, University of Saskatchewan) ;
- ICK-DONG YOO (Genetic Engineering Research Institute, Korea Institute of Science and Technology) ;
- YOUNG-HO YOON (Department of Animal Sciences, Chung-Ang University) ;
- FRANK L GRAHAM (Departments of Biology and Pathology, McMaster University) ;
- LORNE A. BABIUK (Veterinary Infectious Disease Organization, University of Saskatchewan)
- 발행 : 1992.09.01
초록
The hemagglutinin-esterase glycoprotein (HE) gene of bovine coronavirus, coupled with a simian virus 40 early promoter and polyadenylation signal, was inserted into a human adenovirus transfer vector. The transfer vector was used to co-transfect 293 cells along with adenovirus genomic DNA. The hemagglutinin-esterase transcription unit was rescued into the adenovirus genome by homologous in vivo DNA recombination between the vector plasmid DNA and the adenovirus genomic DNA, and a recombinant adenovirus was isolated by several rounds of plaque assays. Thus the recombinant adenovirus carries the hemagglutinin-esterase gene in the early transcription region 3 (E3) of the adenovirus genome in the parallel orientation to the E3 transcription. The recombinant adenovirus synthesized the HE polypeptide in HeLa cells as demonstrated by immunoprecipitation with anti-coronavirus rabbit antisera. The recombinant HE polypeptide could be labelled by
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