The Effect of Choline Deficiency on Lipid Metabolism in Chicks

콜린 결핍이 병아리의 지질대사에 미치는 영향

In the experiment in which young chicks were fed the semisynthetic diet devoid of choline or the same diet with butanolamine supplementation, the weight gain was decreased significantly accompanied by the reduction of feed consumption in choline deficient chicks as compared to control chicks. However, the overall effects of choline deficiency on the relative liver weight, lipid contents of liver, and plasma lipid and lipoprotein levels were not observed, nor was the response to choline deficiency on the incorporation of $^{14}$ C-oleic acid into lipids in the liver microsomes. When hyperlipidemia was induced by estrogen treatment, the liver lipids, as well as relative liver weight, showed a tendency to be increased only in the chicks fed the semisynthetic diet devoid of choline with butanolamine supple-mentation. And the magnitude of elevation of VLDL lipids by estrogen treatment was the lowest in the above group. These results indicated that young chicks were not able to synthesize considerable choline for normal growth ; nevertheless, the release of VLDL by hepatocytes was performed normally. But it was also implied that there might be some problems of VLDL release under the condition of hyperlipidemia in chicks in choline deficiency accelerated by butanolamine supplementation.

1주령 병아리에 무콜린 식이를 급여하거나 무콜린 식이에 butanolamine을 첨가 급여하여 콜린 결핍을 심화시켜, 콜린 결핍이 지질대사에 미치는 영향을 알아보았다. 그 결과 콜린 결핍은 간장중량, 혈장 지질 및 지단백 수준에 유의적인 영향을 나타내지 않았다. 또한 간세포의 소포체에서 $^{14}$ C-oleic acid의 지질 혼입율에도 유의적인 영향을 끼치지 않았다. 한편 estrogen을 투여하여 고지혈증을 야기 시킨 경우 콜린 결핍군의 VLDL상승 정도가 낮은 경향을 나타내었고 비간장 무게와 간장의 지질함량은 증가하는 경향을 나타내었다. 한편 콜린 결핍은 병아리의 성장을 저해하였다 성장에 필요한 콜린을 충분히 합성하지 못하는 어린 병아리에 콜린 결핍을 야기 시켜 얻어진 이러한 결과는 간세포에서의 VLDL방출에 콜린이 크게 작용하지 않음을 시사하여 주었다. 그러나 고지혈증이 유발된 상황에서 극심한 콜린 결핍은 VIDL방출에 약간의 영향을 끼칠 수도 있음을 나타내었다.