Korean Journal of Microbiology (미생물학회지)
- Volume 30 Issue 4
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- Pages.299-304
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- 1992
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Purification and Characterization of an Intracellular Protease form Pseudomonas carboxydovorans DSM 1227 Grown on Carbon Monoxide
- Ho, Bae-Ki (Department of Biology, College of Science, Yonsei University) ;
- Kim, Young-Min (Department of Biology, College of Science, Yonsei University)
- Published : 1992.08.01
Abstract
An intracellular protease form cells of Pseudomonas carboxydovorans DSM 1227 grown on carbon monoxide was purified 57-fold in six steps to homogeneity with a yield of 4.3% using azocoll as a substrate. The molecular weight of the enzyme was determined to be 150,000. Sodium dodecyl sulfate-gel electrophoresis revealed the purified enzyme to be a dimer with two identical subunits of molecular weight 72,000. The enzyme was stimulated by