YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
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Screening of Methotrexate-Resistant Strains with High Thymidylate Synthase Activity

티미딜산 생성효소 활성이 높은 메토트렉세이트-내성 균주의 검색

  • Kwak, In-Young (Department of Genetic Engineering, Pai-Chai University) ;
  • Lee, Jong-Soo (Department of Genetic Engineering, Pai-Chai University)
  • 곽인영 (배재대학교 이공대학 유전공학과) ;
  • 이종수 (배재대학교 이공대학 유전공학과)
  • Published : 1992.08.29
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Abstract

Thymidylate synthase activity from extracts of various methotrexate-resistant strains was measured by spectrophotometric assay. Methotrexate-resistant strains of Lactobacillus, Pseudomonas sp., Micrococcus sp. HS-1, Klebsillela pneumonae, Cellulomonas fimi and Serratia marcescens elevated thymidylate synthase levels, especially, Pseudomonas sp. KL-9 resistant to $10^{-9}M$ methotrexate have a 156-fold increase in thymidylate synthase, which suggests that Pseudomonas sp. is a convenient source of thymidylate synthase. Several methotrexate strains of yeast were tested, however, their enzyme activity was generally lower than that of bacteria tested.

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