YAKHAK HOEJI (약학회지)
- Volume 36 Issue 2
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- Pages.180-187
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- 1992
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- 0377-9556(pISSN)
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- 2383-9457(eISSN)
Oxyradical Formation during the Hepatocarcinogenesis in Rat
흰쥐 간발암화 과정에서의 산소유리기의 동태
- Kim, Hyoung-Chun (College of Pharmacy, Kangweon National University) ;
- Chun, Wan-Jhoo (College of Pharmacy, Kangweon National University) ;
- Lee, Hyun-Woo (College of Pharmacy, Kangweon National University) ;
- Kwon, Myung-Sang (College of Animal Agriculture, Kangweon National University) ;
- Song, Kye-Yong (College of Medicine, Chung Ang University) ;
- Jhoo, Wang-Kee (College of Pharmacy, Kangweon National University)
- 김형춘 (강원대학교 약학대학) ;
- 전완주 (강원대학교 약학대학) ;
- 이현우 (강원대학교 약학대학) ;
- 권명상 (강원대학교 축산대학) ;
- 송계용 (중앙대학교 의과대학) ;
- 주왕기 (강원대학교 약학대학)
- Published : 1992.04.29
Abstract
This study investigated the hypothesis that carcinogen-induced elevation of oxyradical during the hepatocarcinogenesis in rat. The hepatic preneoplastic lesions in the Spraque-Dawley rats were induced by the carcinogen treatment such as diethylnitrosamine(DEN) and acetylaminofluorene(AAF) in combination with partial hepatectomy(PH). The liver sample was taken at 2, 6, 10 and 16 months after carcinogen treatments followed by PH. Carcinogen treatments initially increased the indices of oxidative damage(activities of xanthine oxidase and production rates of superoxide anion, microsomal hydrogen peroxide, hydroxyl radical) in the liver compared to PH groups. However, cytosolic hydrogen peroxide did not change significantly throughout the full time period. Of hydrogen peroxide scavenger, the catalase was remained lower than PH groups, whereas the peroxidase was increased after carcinogen treatments. Morphologically, the immunohistochemical analysis with glutathione-S-transferase of a placenta form(GSTP) antibody was used to detect the induction of preneoplastic nodules. During the hepatocarcinogenesis, both production rate of hydroxyl radical and activity of glutathione-S-transferase(GST) markedly increased with the appearance of the preneoplastic nodule. These results indicated that the hydroxyl radical of reactive oxygen species seemed to have a major influence on the hepatocarcinogenesis and the effect of time after removal of the carcinogen also appeared to be highly critical in the hepatocarcinogenesis.
Keywords
- Reactive oxygen species;
- hydroxyl radical;
- glutathione-S-transferase;
- hepatocarcinogenesis;
- partial hepatectomy;
- immunohistochemical analysis;
- pre neoplastic nodule