Gas Chromatographic Profiling for the Screening of Candida tropicalis Mutant Producing Tridecanedioic Acid

Gas Chromatographic Profiling법을 이용한 Tridecanedioic Acid를 생산해내는 Candida tropicalis Mutant의 탐색연구

  • Kim, Jung-Han (Department of Food Engineering, College of Engineering, Yonsei University) ;
  • Lee, Sang-Jun (Department of Food Engineering, College of Engineering, Yonsei University) ;
  • Park, Hyoung-Kook (Department of Food Engineering, College of Engineering, Yonsei University) ;
  • Kim, Kyoung-Rae (College of Pharmacy, Sungkyunkwan University)
  • 김정한 (연세대학교 공과대학 식품공학과) ;
  • 이상준 (연세대학교 공과대학 식품공학과) ;
  • 박형국 (연세대학교 공과대학 식품공학과) ;
  • 김경례 (성균관대학교 약학대학 제약학과)
  • Published : 1991.04.01

Abstract

Tridecanedioic acid (DC-13), starting material of the valuable musk ethylene brassylate, was obtained from n-tridecane by the Candida tropicalis mutant. The mutants were first obtained from primary screening step using the selective medium and then solid phase extraction sampling method was used for the selective isolation of organic acids from the cultured media of mutants. The resulting acids were directly converted to volatile tert-butyldimethyl silyl delivatives, which were then analyzed by gas chromatography. The efficient GC profiling method was used for the rapid identification of the mutant producing DC-13 in large quantity, and for the optimization of the culture conditions of mutant. The optimal culture conditions were found as follows: pH 8.0, 30$^{\circ}C$, 250rpm, 48hour of culture and $(NH_4)_2HPO_4$ as nitrogen source.

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