Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 19 Issue 2
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- Pages.171-178
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- 1991
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Continuous Production of Cyclodextrin in Two-Stage Immobilized Enzyme Reactor Coupled with Ultrafiltration Recycle System
2단계 고정화 효소반응기를 활용한 Cyclodextrin의 연속생산
- Lee, Yong-Hyun (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ;
- Lee, Sang-Ho (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ;
- Han, Il-Keun (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University)
- Published : 1991.04.01
Abstract
The two-stage enzyme reactor, packed with cyclodextrin glucanotransferase (CGTase) immobilized on Amberite IRA 900, coupled with ultrafiltration membrane was investigated for continuous production of cyclodextrin (CD). 5% (w/v) of soluble starch was partially cyclized, in the 0.1 l first-stage immobilized enzyme reactor, up to CD conversion yield of 10% (w/w) at retention time of 0.56hr and 1.5 units of immobilized CGTase/1g of carrier. In the second stage main immobilized enzyme reactor capacity of 1.5 l, the maximum CD conversion yield of 39% (w/v) was achieved at retention time of 2.8hr and 0.47 unit of CGTase/1 g of carrier. Unreacted residual dextrin was fractionated with ultrafiltration membrane, and then, recycled into the second-stage main bioreactor to increase the CD conversion yield. The most suitable membrane size and the volume concentration ratio (concentrate: filterate) for recycling of unreacted residual dextrin were found to be 5K dalton and 4:6, respectively. CD conversion yield was increased about 3~4% upon co-immobilization of pulluanase along with CGTase. Spent Amberite IRA 900 can be reutilized consecutively more than 3 times for immobilization of CGTase after regeneration.
Keywords
- Immonilized enzyme reactor;
- cyclodextrin glucanotransferase;
- cyclodextrin production;
- ultrafiltration membrane