Journal of Microbiology and Biotechnology
- Volume 1 Issue 1
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- Pages.54-62
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- 1991
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Evaluation of Immobilization Methods for Cyclodextrin Glucanotransferase and Characterization of its Enzymatic Properties
- Lee, Sang-Ho (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ;
- Shin, Hyun-Dong (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ;
- Lee, Yong-Hyun (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University)
- Published : 1991.03.01
Abstract
Cyclodextrin glucanotransferase(CGTase) derived from Bacillus macerans was immobilized by (1) covalent linkage on chitosan and chitin with glutaraldehyde, (2) adsorption on DEAE-cellulose and Amberite IRA 900 after succinylation, and (3) entrapment on alginate and polyacrylamide by cross linking. Adsorption on Amberite IRA 900 and covalent linking on chitosan were identified to be the most suitable immobilization methods considering the yield of activity and stability of immobilized CGTase. The enzymatic properties of immobilized CGTase were investigated and compared with those of the soluble CGTase. Thermal stability of CGTase immobilized on chitosan was increased from 50 to
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