옥수수 배의 중성 지질의 추출을 위한 7종 용매의 비교 연구

Comparative Studies of Seven Solvents for the Extraction of Neutral Lipid in Corn Embryo

옥수수 배를 benzene(BZ), n-hexane(HX), petether(PE), tricholoroethhylene(TE), chloroform-methanol(2:1, v/v) (CM), dichloromethane-methanol(2:1, v/v) (DM), hexane-diethyiether(5:1, v/v) (HD)의 7종 용매로 조지질을 추출하여 SACC법으로 비극성과 극성지질을 분리한 후 각각의 지질 조성을 TLC scanner로 측정하고 GC로 지방산 조성을 조사한 결과를 검토하였다. 조지질 추출양은 DM에서 가장 많고 다음은 CM이며 중성지질은 HX, BZ, HD에서 높은 반면 PE, DM에서 낮았으며 함량비에서는 DM과 CM에서 높으며, 당지질은 DM을 사용했을 때, CM, PE, HX 등보다 높고 HD에서 가장 낮았고 인지질은 CM에서 가장 높게 나타났다. 중성지질의 분별정량에서 triglyceride는 DM에서 높고 PM에서 낮은 편이며 1, 3-diglyceride는 CM 에서 높고 DM에서는 낮았다. 총지질의 지방산 조성은 linoleic acid가 주요 성분이며 이는 CM에서 높은 편이며 oleic acid는 PE, HD등을 사용했을 때 CM보다 높고 중성지질의 지방산 조성도 linoleic acid가 가장 많고 BZ에서 높게 나타났으며, PE, CM, HD 등에서 낮고 oleic acid는 비슷하나 DM, BZ에서는 낮다. Palmitic acid는 CM, PE에서 가장 높게 나타났다.

The comopositon of lipids extracted from corn embryo with various solvents were analyzed. The solvents for the extraction were benzene(BZ), n-hexane(HX), pet. ether(PE), trichlorethylene (TE), chloroform-methoanol(2:1, v/v) (CM), dichlormethane - methanol(2:1, v/v)(DM) and hexanediethyl ether(5:1, v/v)(HD). The lipids were than fractinated by silicis acid column chromatography(SACC) into three lipid fractions. The Neutral lipid fractons were further separated by thin layer chromatography(TLC) and the individual lipid spots were quantitatived by TLC scanner. And then the fatty acid compositions of total lipids and neutral lipids were determined by gas chromatography(GC). Crude oil contents of corn embryo were most efficient by using DM, CM and neutral lipid was extracted much HX, BZ, HD systems than did PE, DM, DM an CM were most efficient solvent systems for extracting glycolipid and phospholipid. The major component of the neutral lipid fractions was found to be triglyceride, and it was superior DM to PE. Linoleic acid was the predominant fatty acid in the total lipids, and it was most efficient with BZ and TE. The major fatty acid in neutral lipids was also linoleic acid and it was superior BZ to PE, CM, HD and oleic acid was similar to seven solvents and palmitic acid was found much superior in using CM.