Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Identification of Malonate-specific Enzymes, Malonyl-CoA Synthetase and Malonamidase, in Rhizobia

Rhizobia에서 Malonyl-CoA synthetase와 Malonamidase의 확인

  • Kim, Yu-Sam (Department of Biochemistry, College of Science, Yonsei University) ;
  • Chae, Ho-Zoon (Department of Biochemistry, College of Science, Yonsei University) ;
  • Lee, Eun (Department of Chemistry, Seoul National University) ;
  • Kim, Yong-Sung (Department of Chemistry, Seoul National University)
  • 김유삼 (연세대학교 이과대학 생화학과) ;
  • 채호준 (연세대학교 이과대학 생화학과) ;
  • 이은 (서울대학교 자연대학 화학과) ;
  • 김용성 (서울대학교 자연대학 화학과)
  • Published : 1991.03.01
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Abstract

Two malonate-specific enzymes, malonyl-CoA synthetase and malonamidase, were found in free-living cultures of Rhizobium japonicum, Rhizobium meliloti, and Rhizobium trifolii, that infect plant roots where contain a high concentration of malonate. Malonyl-CoA synthetase catalyzes the formation of malonyl-CoA, AMP, and PPi directly from malonate, coenzyme A, and ATP in the presence of $Mg^{2+}$ Malonamidase is a novel enzyme that catalyzes hydrolysis and malonyl transfer of malonamate, and forms malonohydroxamate from malonate and hydroxylamine. Both enzymes are highly specific for malonate. These results show that Rhizobia have enzymes able to metabolize malonate and suggest that malonate may be used in symbiotic carbon and nitrogen metabolism.

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