Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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QUANTITATION OF BARBITURATES IN URINE BY GC/MS AND ITS COMPARISON TO FLUORESCENCE POLARIZATION IMMUNOASSAY

  • Choo, Hea-Young (Dopin Control Center, Korean Advanced Institute of Science and Technology) ;
  • Park, Jeongeun (Dopin Control Center, Korean Advanced Institute of Science and Technology) ;
  • Park, Myung-Ja (Dopin Control Center, Korean Advanced Institute of Science and Technology)
  • Published : 1991.06.01
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Abstract

Barbiturates are commonly abused tranquilizer and a rapid method to determine these drugs in biological samples is needed. In this study, was screened barbiturates in urine specimens by the fluorescence polarization immunoassay method(FPIA) and the positive samples were confirmed and identified by the more definitive GC/MS method. Fifteen positive smples which have barbiturate values higher than 0.5 ng/ml were analyzed by the GC/MS method. Eight samples were identified as phenobarbital and five samples were identified as crotilbarbitone.

Keywords

References

  1. J. Chrom. v.190 Analysis of trace amounts of barbiturates in Saliva Dilli,S.;Pillai,D.
  2. Anal. Chem. v.24 Determination of barbiturates Goldbaum,L.R.
  3. Clin. Chem. v.27 no.9 Fluorescence polarization immunoassay. III. An automated system for therapeutic drugs determination Jolley,M.E.;Struope,S.D.;Schwenzer,K.S.;Wang,C.J.;Lu-Steffes,M.;Hill,H.D.;Popelka,S.R.;Holen,J.T.;Kelso,D.M.
  4. J. Anal. Tox. v.2 A simultaneous high-performance liquid chromatographic analysis of the most common acticonvulsants and their metabolites in the serum Kabra,P.M.;McDonald,D.M.;Marton,L.J.
  5. J. Pharm. Sci. v.69 Simultaneous analysis of phenobarbital and p-hydroxy-phenobarbital in biological fluids by GLC-chemical Ionization lonization mass spectrometry Patel,I.H.;Levy,R.H.;Neal,J.M.;Trager,W.F.
  6. J. Chromatogr. v.105 Gas chromatographic methods for the microdetermination of barbiturates in blood using a nitrogen-selective flame ionization detector Prorchik,B.H.
  7. Clin. Tox. v.8 Single extraction of GLC analysis of six commonly prescribed antiepileptic drugs Ritz,D.P.;Warren,C.G.
  8. Anal. Chem. v.45 Rapid determination of barbiturates by Gas chromotography-Mass spectrometry Skinner,R.F.;Gallaher,E.G.;Predmore,D.B.
  9. J. Pharm. Sci. v.66 Determination of pentobarbital in serum by electron-capture GLC Sun,S.R.;Chun,A.H.C.
  10. J. Chromatogr. v.258 Analysis of barbiturates in plasma and urine using gas chromatography without prior derivatization Villen,T.;Petters,I.