Immobilization of ${\alpha}-amylase$ on Polyethylene Glycol Grafted Polyurethane Film

폴리에틸렌글리콜이 그라프트된 폴리우레탄 막에 ${\alpha}$-아밀라제의 고정화

${\alpha}-amylase$ was immobilized on the surface of polyethylene glycol(M.W. 2000) grafted polyurethane film using diisocyanate in an attemp to develop enzyme immobilized polymeric materials. The surface morphology of the modified polyurethane film was examined by SEM. Effects of pH and temperature on the activity of the immobilized ${\alpha}-amylase$ were investigated. The optimal pH range of the activity was $7.0{\sim}7.5.$ The immobilized ${\alpha}-amylase$ demonstrated high thermal stability and maintained consistent activity during long-term storage.