Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Study on Pregnancy and Viability of Frozen-Thawed Human Embryos by Cryopreservation : DMSO as Cryoprotectant

동결보존에 의한 인간배아의 생존률과 임신에 관한 연구

  • Lee, Ho-Joon (IVF Research Laboratory, Department of Obstetrics and Gymecology, Cheil General Hospital) ;
  • Lee, Seung-Jae (IVF Research Laboratory, Department of Obstetrics and Gymecology, Cheil General Hospital) ;
  • Roh, Sung-Il (IVF Research Laboratory, Department of Obstetrics and Gymecology, Cheil General Hospital) ;
  • Paik, Hye-Ran (IVF Research Laboratory, Department of Obstetrics and Gymecology, Cheil General Hospital) ;
  • Kim, Moon-Kyoo (Department of Biology, College of Natural Sciences, Hanyang University)
  • 이호준 (제일병원 산부인과 체외수정 연구실) ;
  • 이승재 (제일병원 산부인과 체외수정 연구실) ;
  • 노성일 (제일병원 산부인과 체외수정 연구실) ;
  • 백혜란 (제일병원 산부인과 체외수정 연구실) ;
  • 김문규 (한양대학교 자연과학대학 생물학과)
  • Published : 1990.11.30
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Abstract

This study was done to verify factors affecting viability after cryopreservation and pregnancy rate after frozen-thawed embryo transfer into uterus. Embryos were cryopreserved slow freezing and slow thawing and used DMSO as cryoprotectant. The results were to follows. 1. Viability of frozen-thawed embryos were 75.5% (94/105), which compared with viability of embryos according to cell stage, $2{\sim}5$ cell was 68.4% and $6{\sim}16$ cell 80.4% were significant differences (p<0.05). 2. No significant difference in duration of cryopreservation on effects affecting pregnancy rate was observed. 3. Number of embryo transfered into uterus was significant differences (p<0.05). 4. Four pregnancies resulted following replacement of 35 frozen-thawed.

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