Abstract
This study was performed for assessing carcinogenicity of several synthetic hormones; Diethylstilbesterol (DES), 17-ethinylestradiol ($EE_2$) and Bovine somatotrophin (BST). Six weeks old F344 rats were divided into five groups and given an intraperitoneally injection of 200 mg of diethylnitrosamine (DENA). At two week after beginnig of experiment, DES, $EE_2$, BST. Phenobarbital were administered to group 1, 2, 3 and 4 respectively, group 4 is positive control and group 5 is negative control. At the same time, all groups received a single i.p. injection of D-galactosamine at a dose of 300 mg/kg and underwent 2/3 partial hepatectomy at week 5. All rats were sacrificed at the end of week 8 for assessment of liver lesion development. The liver was processed for immunohistochmical staining for GST-P and quantitatively analyzed by image analyzer. It was concluded that two synthetic estrogen hormones (DES, $EE_2$) was different significantly (p < 0.01) but BST was not different as compared with control group. Therefore, we thought that DES, $EE_2$ was promoting effects and BST was not in rat hepatocarcinogenesis.
이 연구는 수입 축산식품중에 잔류해서 사람에게 해를 줄 수 있는 여러 합성 홀몬의 발암성을 알아보기 위하여 수행한 연구이다. 6주령의 F344 랫드를 5군으로 나누어 발암유발물질인 diethylstilbesterol(DENA)를 체중 kg당 200 mg을 복강 내 투여하고, 시험게시 제2주에서 시험종료시까지 제 1, 2, 3,군에 각각 diethylstilbesterol(DES), ethynylestradio($EE_2$), bovine somatotrophin(BST)을 투여하고, 제 4군에는 발암촉진이 잘 알려진 phenobabital을 투여하였으며, 제 5군은 대조군으로 기초사료만을 급여하였다. 동시에 모든군의 랫드는 D-galactosamine을 체중 kg 당 300 mg을 복강내로 1회 투여하였으며, 간변화의 증폭을 위하여 2/3 간부분 절제를 시험개시 제 5주에 실시하였다. 시험종료시 부검후 랫드의 간장은 GST-P에 대한 면역조직화학적 염색을 실시하여 발암성을 검색하였다. 두개의 합성 에스트로젠 홀몬($EE_2$, DES)은 대조군과 비교하였을 때 발암성이 있는 것으로 생각되어지고, BST는 발암성이 없는 것으로 생각된다.
