Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 18 Issue 6
- /
- Pages.578-584
- /
- 1990
- /
- 1598-642X(pISSN)
- /
- 2234-7305(eISSN)
Produ cti on of Cyclomaltodextrin from Bacillus stearothermophilus
Bacillus stearothermophilus에 의한 Cyclomaltodextrin Glucanotransferase의 생산
Abstract
A microorganism capable of producing high level of extracelluar cyclomaltodextrin glucanotransferase(EC 2.4.1.19; CGTase) was isolated ’rom soil. The isolated strain No. 239 was identified as Bacillusstearothermophilus. The maximal CGTase production (about 7.0 unitslml) was observed in medium containing2% soluble starch, 0.5% defatted soybean meal, 0.1%
토양시료로부터 CGTase를 생산하는 중성의 호열성 미생물을 conogo red가 포함된 배지를 이용하여 분리하고, 분리균주의 형태학적, 생리학적 성질을 조사한 결과 Bacillus stearothermophilus No.239로 동정되었다. 초기 pH가 7.0인 soluble starch 2, defatted soybean meal 0.5,