• Title/Summary/Keyword: cycolmaltodextrin glucanotransferase

Search Result 1, Processing Time 0.016 seconds

Produ cti on of Cyclomaltodextrin from Bacillus stearothermophilus (Bacillus stearothermophilus에 의한 Cyclomaltodextrin Glucanotransferase의 생산)

  • 황진봉;김승호;이태경;양한철
    • Microbiology and Biotechnology Letters
    • /
    • v.18 no.6
    • /
    • pp.578-584
    • /
    • 1990
  • A microorganism capable of producing high level of extracelluar cyclomaltodextrin glucanotransferase(EC 2.4.1.19; CGTase) was isolated ’rom soil. The isolated strain No. 239 was identified as Bacillusstearothermophilus. The maximal CGTase production (about 7.0 unitslml) was observed in medium containing2% soluble starch, 0.5% defatted soybean meal, 0.1% NaH_2PO_4.2H_2O$ and 0.015% $ CaC_l2 $ with initial pH 7.0. The strain was cultured at $55^{\circ}C$ for 48 hr with reciprocal shaking. At 0.83% substrated concentration potato starch was the optimum substrate with 50.1% conversion to cyciodextrin (CD)after the reaction at $65^{\circ}C$ for 24 hr (CGTase 10 unitlg starch). Using soluble starch as substrate (5% substrate concentration, CGTase 10 unitlg starch), the maximum conversion of 40% was obtained at11 hr reaction, and the ratio of $\alpha-, \beta-$ and $\gamma$-CD production at this time were 1.0:1.3:0.4, respectively., respectively.

  • PDF