IAA수준의 조절에 미치는 과산화수소의 영향

Involvement of hydrogen peroxide in the regulation of IAA level in plants

  • Park, Ro-Dong (Department of Agricultural Chemistry, Chonnam National University) ;
  • Kim, Jeong-Bong (Department of Agricultural Chemistry, Chonnam National University) ;
  • Park, Chang-Kyu (Department Agricultural Chemistry, Seoul National University)
  • 발행 : 1990.06.30

초록

저온에서 식물체중에 축적되는 과산화수소의 생리적 기능을 IAA수준의 조절과 관련하여 연구하였다. 과산화수소는 10mM농도에서 완두의 발근과 귀리 초엽의 신장을 억제하였으며 IAA의 호르몬 효과도 억제하였다. 이 억제효과는 catalase를 첨가하면 해제되었다. 유리의 IAA는 과산화수소와 반응하여 Salkowski시약에 민감하지 않은 어떤 복합체를 형성하여 과산화수소에 의하여 불활성화되는 것처럼 보였다. 그러므로 저온에서 식물체내에 축적된 과산화수소는 IAA를 불활성화하여 세포의 유용한 IAA수준을 하향조절하였을 것으로 여겨졌다.

The role of hydrogen peroxide which is accumulated in plants under low temperature has been studied with respect to the regulation of physiological IAA level. At 10 mM of $H_2O_2$, accelerating effects of IAA on the elongation of Avena coleoptiles and the root Initiation of pea cuttings have been greatly inhibited. These inhibitions were reversed by introduction of catalase. The reaction of free IAA with Salkowski reagent was inhibited in the presence of $H_2O_2$, but that of IAA-glutamic acid was not, suggesting the inactivation of free IAA by $H_2O_2$. The data support that increase in the content of hydrogen peroxide under low temperature partially down-regulates the available IAA through inactivation of IAA.

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