Effect of Some Parameters for Selecting the High Kasugamycin Producing Mutants

고수율 Kasugamycin 생산 변이주의 선발시 여러 인자들의 효과

  • 김윤정 (한국화학연구소 생물공학연구실) ;
  • 이상한 (한국화학연구소 생물공학연구실) ;
  • 손광희 (한국화학연구소 생물공학연구실) ;
  • 복성해 (한국화학연구소 생물공학연구실)
  • Published : 1989.04.01

Abstract

Effects of the inoculum size of testing organism and pH of the plate and the concentration of agar were investigated for the selection of high kasugamycin producing mutants of Streptomyces kasugaensis ATCC 15114. For the detection of high kasugamycin-producing mutants, both concentrations of agar and test organism were optimized at the concentrations of 2% and 0.35 (A$_{550}$), respectively. The pH 7 was optimum for both growing the testing organism, Pseudomonas fluorescens IFO 12180, and for obtaining more promising mutant strains of S. kasugaensis. Under these conditions, mutants had been isolated which, tested later in liquid cultures, gave higher kasugamycin yields than that of the parent strain.

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