An Effect of Levamisole on the Chemical Carcinogenesis in the Submandibular Salivary Gland of Rats

Levamisole이 백서 악하선에서의 화학적 발병현상에 미치는 영향

The purpose of this study is to evaluate an effect of levamisole on the chemical crcinogenesis in the submandibular salivary gland of rats through histopathologic observation. 60 male Sprague Dawley rats were employed in this study, divided into one control and two experimental groups. An pellet of 5 mg of 9, 10-dimethyl-1,2-benzathracene(DMBA) powder was implanted into submandibular salivary gland of each animal among 20 in control. And each animal among 20 in experimental group 1 received 0.7 mg of levamisole hydrochloride orally every day starting at the beginning of the fifth week after DMBA implantation under the same methods as in control. And each animal among 20 in experimental group 2 received the same treatment as in control at the beginning of the fifth week after oral administration of levamisole hydrochloride under the same method as experimental group 1. Each 5 animals in control at the end of 2nd, 4th, 6th 8th, week after experiments, and each 10 animals in experimental group 1,2 at the end of 6th, 8th week after experiments were sacrificed at random. Also the specimens from experimental sites of submandibular salivary glands were routinely processed for histopathologic observation under Hematoxilin-eosin(H-E) staining. The obtained results were as follows : 1. In control, generally, the glandular ductal cell showed the tendency of dysplasia or malignancy with progression of experiment. 2. In experimental group 1, generally, the dysplasia or malignancy of the glandular ductal cell was less prominent than in control, while the lymphocyte infiltration and fibrosis were prominent. 3. In experimental group 2, generally, the dysplasia of the glandular ductal cell was significantly less prominent than in control, while the fibrosis was prominent. 4. Under above results levamisole was thought to delay or prevent the chemical carcinogenesis in the submandibular salivary gland.