Isolation of Phenolic Antioxidant Components from Panax ginseng

인삼으로부터 페놀성 항산화 성분의 분리

To isolate new antioxidants from Panax ginseng, methanol extract of Panax ginseng was fractionated into several subfractions and their antioxidant activities were measured by using ${\alpha}$, ${\alpha}-diphenyl-{\beta}-picrylhydrazyl$(DPPH). Four compounds were isolated from two subfractions that showed high DPPH quenching activities by silica gel, ${\mu}$ Bondapak $C_{18}$ or Sephadex LH-20 column chromatography. The four compounds were confirmed to be phenolic substances by color reaction and UV spectral analysis.

인삼중 새로운 항산화 성분을 분리하기 위하여 인상의 메탄올 추출물을 여러 성분 분획으로 나눈 다음 ${\alpha}$, ${\alpha}-diphenyl-{\beta}-picrylhydrazyl(DPPH)$을 이용하여 각각의 항산화 활성을 측정하였다. DPPH 소거 활성이 비교적 큰 두 분획을 silica gel, ${\mu}-Bondapak$ $C_{18}$ 또는 Sephadex LH-20 column chromatograph하여 4중의 단일성분을 분리하였고 이들은 정색반응 및 UV분석을 통해 모두 페놀성 성분으로 확인되었다.