Identification of Ginseng Sapogenin and Quantitative Determination of $Ginsenoside-Rb_1$ from Crude Drug Preparation Containing Bupleuri Radix

시호(柴胡)함유 생약제제(生藥製劑)중 인삼(人蔘) Sapogenin의 확인 및 $Ginsenoside-Rb_1$의 분리 정량

From crude drug preparation(Soshiho-Tang) ginseng sapogenins were identified by TLC and $ginsenoside-Rb_1$ was determined quantitatively by HPLC. Panaxadiol, pandaxatriol, acid-hydrolysates of ginseng saponin, were identified by TLC with benzene/acetone(4 : 1, v/v). Rf values of which were measured as 0.26 and 0.14, respectively. The content of $ginsenoside-Rb_1$ was determined by HPLC on $Lichrosorb-NH_2$ column with $CH_3CN/H_2O/n-BuOH$(80 : 20 : 10, v/v). Its recovery rate in the extract granules, was as relatively low as $19.8{\pm}1.4%$ compared to the content in raw red ginseng.