한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)
- 제21권2호
- /
- Pages.85-96
- /
- 1988
- /
- 0374-8111(pISSN)
- /
- 2287-8815(eISSN)
The Proteinase Distributed in the Intestinal Organs of Fish 3. Purification and Some Enzymatic Properties of the Alkaline Proteinases from the Pyloric Caeca of Skipjack, Katsuwonus vagans
- PYEUN Jae-Hyeung (Department of Nutrition and Food Science, National Fisheries University of Pusan) ;
- KIM Hyeung-Rak (Department of Food Science, Louisiana State Univ.) ;
- HEU Min-Soo (Department of Nutrition and Food Science, National Fisheries University of Pusan)
- 발행 : 1988.05.01
초록
Purification and some properties of alkaline proteinases in the pyloric caeca of skipjack, Katsuwonus vagans, were investigated. Four alkaline proteinases, temporarily designated proteinases I, II, III and IV, were identified from the tissue extract of the pyloric caeca by ammonium sulfate fractionation, DEAE-Sephadex A-50 chromatography, and Sephadex G-100 and G-200 gel filtration. Result of disc-polyacrylamide gel electrophoretic analysis showed that the purified proteinases II and III were homogenous with the yields of
키워드