Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 16 Issue 3
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- Pages.182-186
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- 1988
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Isolation and Characterization of Intraspecific Complementing Fusants of Penicillium verruculosum
Penicillium verruculosum의 종내원형질 융합체의 분리 및 특성
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Chung, Ki-Chul
(Institute of Biotechnology, Chonnam National University)
;
- Park, Chang-Ryeol (Institute of Biotechnology, Chonnam National University) ;
- Suk Bai (Institute of Biotechnology, Chonnam National University) ;
- Chun, Soon-Bai (Institute of Biotechnology, Chonnam National University) ;
- Kim, Ki-Chung
- Published : 1988.06.01
Abstract
The possibility of strain improvement of cellulolytic fungus, Penicillium verruculosum via protoplast fusion was investigated. The cellulolytic activities of the six fusants, finally selected for their hyper-cellulolytics were 2 times of those of wild type and 1.2 to 4.4 times of those parental auxotrophs. It was confirmed that the nuclear fusion occurred in fusants by their DNA contents and nuclear staining with Giemsa. It was also found that the fusants were aneuploids, and their genetic stability was demonstrated from the subculture for four months.
원형질체 융합기술에 의한 Penicillium verruculosum 균주개량 가능성을 검토하였다. 영양요 구변이주간의 원형질 융합체 165주중 cellulase 고생산 융합체로 선별된 6주의 cellulase 활성은 야생주에 비해 2배이상, 모균주에 비해 1.2-4.4배의 증가를 나타냈다. Cellulase 고생산 융합체는 유전적 안정성조사, DNA 함량 및 핵염색 비교검토를 통해 핵융합이 일어난 것으로 확인되었고, 핵형이 이수체이며 4 개월 이상의 계대배양 후에도 자연형질 분리가 거의 일어나지 않는 것으로 보아 이들 융합체의 안정성이 매우 높은 것으로 인정되었다.