한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제16권2호
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- Pages.163-167
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- 1988
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Penicillium verruculosum의 종내 원형질체 융합
Intraspecific Protoplast Fusion of Cellulolytic Fungus, Penicillium verruculosum
- Chung, Ki-Chul (Institute of Biotechnology, Chonnam National University) ;
- Park, Chang-Ryeol (Institute of Biotechnology, Chonnam National University) ;
- Suk Bai (Institute of Biotechnology, Chonnam National University) ;
- Chun, Soon-Bai (Institute of Biotechnology, Chonnam National University) ;
- Kim, Ki-Chung
- 발행 : 1988.04.01
초록
Penicillium verruculosum으로부터 유도된 영양요구성 돌연변이주간의 원형질체 융합을 위한 조건을 검토하였다. 20시간 배양한 P. verruculosum의 균사체에 Novozym 234를 처리하여 원형질체를 추출할 수 있었다. 원형질체 생성 최적조건하에서의 원형질체 생성량은 각 영양요구성 변이주의 균사체 400mg 당 2.4~3.0
The conditions for the protoplast fusion of auxotrophic mutants of Penicillium verruculosum were determined. A preparation of commercial enzyme Novozym 234 was used to successfully isolate protoplast from the 20hr old mycelium of P. verruculosum. Under optimal condition, the protoplast yield ranged from 2.4