Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 16 Issue 2
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- Pages.156-162
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- 1988
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Formation and Regeneration of Penicillium verruculosum Protoplasts
Penicillium verruculosum의 원형질체 형성과 재생
- Chung, Ki-Chul (Institute of Biotechnology, Chonnam National University) ;
- Park, Chang-Ryeol (Institute of Biotechnology, Chonnam National University)
- Published : 1988.04.01
Abstract
Optimal conditions for the formation and regeneration of protoplasts of the cellulolytic fungus Penicillium verruculosum were investigated. Among the various commercial cell wall lytic enzymes tested, 0.5%(w/ v) Novozym 234 was the most effective for protoplast formation. The highest yield of protoplast exceeding 4.5
섬유소 분해효소를 생산하는 P. verruculosum F-3 의 세포융합에 관한 연구의 일환으로 균사체로부터의 원형질체 형성과 재생 최적조건을 검토하였다. 세포벽 분해효소로 유효한 각종시판효소제제중 0.5%(w/v) Novozym 234가 가장 효과적이었고 PDY 배지로 3