한국가축번식학회지 (Korean Journal of Animal Reproduction)
- 제12권1호
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- Pages.24-30
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- 1988
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- 1226-5284(pISSN)
X-정자와 Y-정자의 분이에 관한 연구 III. Sephadex Gel 여과에 의한 우정자의 분이
Separation of X- and Y-Bearing Spermatozoa III. Separation of bull spermtozoa by Sephadex Gel Filtration
초록
These experiments were carried out to develop new techniques for In Vitro separatin of X-and Y-bearing spermatozoa. The bull semen was applied to the various Gel-Columns filled with swellen Sephadex G-50 Fine and then elutriated wtih Locke solution (elutriation rate; 1ml/3-4min., 1ml/1-2min.). Elutriated solution was fractionated into 1ml by automatic Fraction Collector and spermatozoa included in each fraction were subjected to the estimation of viability and recovery rate, and to B-body test. The results obtained in these experiments were summarized as follows: 1. When the column size and the elutriation rate were adjusted to 15