Toxicological Research
- 제3권1호
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- Pages.1-8
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- 1987
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- 1976-8257(pISSN)
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- 2234-2753(eISSN)
INDUCTION OF CYTOCHROME P-450 ASSOCIATED MONOOXYGENASE ACTIVITIES BY PHENOBARBITAL AND 3-METHYLCHOLANTHRENE IN PRIMARY CULTURES OF ADULT RAT HEPATOCYTES
- Park, Seong-Kyu (Department of Biological Science and Engineering Korea Advanced Institute of Science and Technology) ;
- Ha, Jong-Ryul (Department of Biological Science and Engineering Korea Advanced Institute of Science and Technology) ;
- Kim, H.M. (Department of Biological Science and Engineering Korea Advanced Institute of Science and Technology) ;
- Yang, K.H. (Department of Biological Science and Engineering Korea Advanced Institute of Science and Technology)
- 발행 : 1987.06.01
초록
In vitro induction of cytochrome 450 associated monooxygenase activities by phenobarbital (PB) and 3-methylcholanthrene (MC) was investigated in primary cultures of adult rat hepatocytes. PB and MC were added to the culture 24 hr after the initial plating of hepatocytes. A signiftcant increase of the activities of 7-ethoxycoumarin 0-deethylase and aryl hydrocarbon hydroxylase were observed in MC and PB treated culture. MC caused about 500% induction of the initial oxidation rates of both enzymes in 48 hr. However the PB maintained both enzyme activities close to the level of freshly isolated hepatocytes. Biphenyl 4-hydroxylase and aminopyrine N-demethylase activities were also induced by MC and PB. But the level of induction was less than that occuring with 7-ethoxycoumarin 0-deethylase and aryl hydrocarbon hydroxylase. When aflatoxin
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