An Improved HPLC Assay Using Hg/Au Electrochemical Detector for S-2-(3-aminopropylamino) ethylphosphorothioate and S-2-(3-methylaminopropylamino) ethylphosphorothioate in Human Plasma

사람혈장중 S-2-(3-Aminopropylamino) ethylphosphorothioate 및 S-2-(3-Methylaminopropylamino) ethylphosphorothioate의 수은/금 전기화학검출기를 이용한 고속액체크로마토 그라프법에 의한 분석

WR 2721 (S-2-(3-aminopropylaminoethylphosphorothioate) is a radioprotective drug that is now undergoing clinical trials in the United States and Japan. a liquid chromatographic electrochemical method for the determination of WR 2721 an WR 3689 [S-2-(3-methylaminopropylamino)ethylphorothioate] in human plasma was developed in this study. This method includes the use of a Hg/Au electrochemical detector and a cyano column for the direct measurement of WR 2721 and WR 3689 in plasma. An analog of WR 2721, WR 149846 was used as an internal standard. WR 2721 and WR 3689 could be well separated from the solvent front, with a mobile phase of acetonitrile-water (20:80), 0.1M acetic acid and 1.2 mM sodium octane sulfonate. This method was shown to be precise. Both intra-day and inter-day results were within 10% CV. Also, sample preparation was fairly simple. Since WR 2721 and WR 3689 were unstable at room temperature, it was essential to use an automatic sample processor with a refrigerator, especially for carrying out routine analyses.