The Journal of the Korean Society for Microbiology (대한미생물학회지)

The Korea Society for Microbiology (대한미생물학회)
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Characterization of Plasmid Encoding a Heat-labile Enterotoxin Originated from an Human Escherichia coli Strain O15: H11.

사람에서 유래한 장독성 대장균의 이열성장독소와 관련된 Plasmid 특성

  • Rhyu, Mun-Gan (Department of Microbiology, Catholic Medical College) ;
  • Kim, Gum-Ryong (Department of Microbiology, Catholic Medical College) ;
  • Chang, Woo-Hyun (Department of Microbiology, College of Medicine, Seoul National University)
  • 유문간 (가톨릭대학 의학부 미생물학교실) ;
  • 김금용 (가톨릭대학 의학부 미생물학교실) ;
  • 장우현 (서울대학교 의과대학 미생물학교실)
  • Published : 1986.03.31
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Abstract

A heat-labile enterotoxin and no heat-stable enterotoxin producing($LT^+ST^-$) plasmid (110 kilobases in size) was isolated from an enterotoxigenic Escherichia coli of human strain O15:H11 and used for analysis of the $LT^+$ deoxyrionucleic acid region using recombinant DNA technology. A DNA segment containing the $LT^+$ DNA region which was one restriction endonuclease BamHl fragment(6.2 kb in size) was joind to a small multicopy plasmid, pUC9. E. coli K-12 strain, JM103 harboring the chimeric plasmid produced greater amounts of LT than did the enterotoxigenic E. coli O15:H11 strain. The BamHl fragment was further digested with various restriction endonucleases and contained no HindIll restriction site which is an essential in $LT^+ST^+$ plasmid. The detailed DNA sequencing of this $LT^+ST^-$ plasmid is required.

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