Parasites, Hosts and Diseases
- Volume 24 Issue 2
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- Pages.171-176
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- 1986
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- 2982-5164(pISSN)
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- 1738-0006(eISSN)
Fractionation of Antigen for ELISA of Bovine Fascioliasis
간질증(肝蛭症)의 효소면역학적(酵素免疫學的) 진단(診斷)을 위한 항원분획(抗原分劃)
- Rhee, Jae-Ku (Department of Veterinary Parasitology, Jeonbug National University) ;
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Baek, Byeong-Kirl
(Department of Veterinary Parasitology, Jeonbug National University) ;
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Lee, John-Hwa
(Department of Veterinary Parasitology, Jeonbug National University)
- Published : 1986.12.31
Abstract
In order to obtain the most specific and sensitive antigen from crude antigens of Fasciola hepatica for the immunodiagnosis of bovine fascioliasis by the enzyme linked immunosorbent assay (ELISA), phosphate buffered saline extract of F. hepatica was prepared. The crude extract was fractionated into 7 antigens using sephadex G-100 column chromatography. Seven fractionated antigens were applied to ELISA, precipitation test and intradermal test, respectively. Results obtained are as follows: 1. The specificity (95% confidence interval in negative sera of bovine fascioliasis; Mean+
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