Effects of Various Carbon Sources and Carbon Catabolite Regulation in Sisomicin Fermentation

Sisomicin발효에 대한 탄소원의 영향과 Glucose에 의한 조절효과

Sisomicin, which is one of aminoglycoside antibiotics, was produced by Micromonospora inyoensis. The effects of carbon sources on sisomicin production were studied in batch cultures. Starch, dextrin and maltose were good carbon sources for the production of sisomicin. However, when glucose was used, the antibiotic productivity decreased significantly due to a carbon catabolite regulation. The carbon catabolite regulation depends mostly on carbon catabolite repression, but not on carbon catabolite inhibition. On the other hand, the growth-production curves of batch cultures show that sisomicin is produced most actively during the idiophase.

Micromonospora inyoensis 균주에 의한 sisomicin의 발효에서 항생물질의 생산성에 대한 여러 가지 탄소원의 영향을 batch culture를 이용하여 검토하였다. Starch, dextrin 및 maltose는 sisomicin의 생산에 좋은 탄소원으로 밝혀졌으나, glucose가 사용되었을 때 sisomicin 생산성은 carbon catabolite regulation에 기인하여 그게 감소되었다. 한편 sisomicin생합성에 대한 carbon catabolite regulation은 catabolite inhibition효과보다 주로 catabolite repression 효과에 좌우되었다.