Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 14 Issue 2
- /
- Pages.175-179
- /
- 1986
- /
- 1598-642X(pISSN)
- /
- 2234-7305(eISSN)
Studies on the Protoplast Formation of Cellulomonas flavigena and its Observations under Scanning Electron Microscope
Cellulomonas flarigena의 원형질체 형성과 주사전자현미경적 연구
- Bae, Moo (Department of Biology, Ewha Womans University) ;
- Lee, Eun-Ju (Department of Biology, Ewha Womans University)
- Published : 1986.04.01
Abstract
In order to develope a protoplast fusion of the genus Cellulomonas having high assimilibility of cellulose, the optimum conditions for the protoplast formation of Cellulomonas flavigena NCIB 12901 was investigated and observed by means of Scanning Electron Microscope. The results suggested that the susceptibility of the cell wall by lysozyme treatment on protoplast formation was considerably depend on the cultural periods of the cells. Cells of C. flavigena at mid exponential phase could more efficiently convert to protoplast cells than those at late exponential phase did. The rate of the protoplast formation was 95%, even though the rate was over 99.9% on counting by indirect method after osmotic shock treatment, when cells of the organism at mid exponential phase were treated with lysozyme (400
Cellulose분해력이 높은 Cellulomonas flavigena NCIB 12901의 원형질체 융합을 위한 원형질체의 형성조건을 조사하여 본 결과 배양기간에 따라 원형질체의 형성율이 매우 달라져 대수증식기 말기보다는 중기때의 세포를 lysozyme처리하는 것이 더 효율적이며 lysozyme 400