非姙娠 및 姙娠한 女子의 血淸蛋白質 패턴의 比較

The Comparison of Protein Patterns of Sera in Non-Pregnant and Pregnant Women

사람의 혈액중 혈청으로써 임신의 진행에 따른 단백질의 변화 양상을 조사함에 있어서 정상인 남자, 비임신 여자, 임신한 여자 및 분만 직후 여자의 혈청을 SDS/polyacrylamide gel 전기영동과 2차원 전기영동 및 아미노산분석을 시도하였다. 각 실험 방법에 의해 분석된 결과는 다음과 같다. 1. SDS/polyacrylamide gel 전기영동에 의해 분자량 약 10,000에서 110,000 dalton 사이의 정상인 남자와 비임신 여자를 비교하였을 때의 숫적 양상은 서로 동일하였으나 bands 3 (22,000 dalton)과 6(39,000)등은 남자에 있어서 여자보다 적은 양으로 나타났다. 비임신 여자와 2주 간격으로 혈액을 채취한 임신한 여자의 혈청단백질 패턴을 비교하면 여러 band들의 양적인 증감이 관찰되었다. 임심한 여자의 단백질은 비임신에 비해 band 3(22,000)이 16주까지는 비임신의 경우와 거의 비슷한 양상을 보였으나 18주부터 임신 말기까지는 단백질의 양이 계속하여 감소되었다. 반면 bands 4(24,000), 9(69,000), 10(70,000), 12(80,000), 13(83,000), 14(86,000), 15(91,000) 및 16(94,000)은 임신이 진행됨에 따라 일반적으로 증가되나 그 이후에는 약간씩 감소하였다. 분만 직후의 혈청단백질의 패턴을 임신 말기와 비교하였을 때 bands 12(80,000), 15(91,000) 및 16(94,000)들의 양이 비교적 증가하였고 여아를 분만한 경우 남아를 분만한 경우보다 bands 4(24,000), 7(51,000) 및 10(70,000)들의 단백질 양이 많음이 관찰되었다. 2. 2차원 전기영동으로 남자와 비임신 여자의 혈청단백질 패턴을 비교하면 spot a(22,000)의 3개의 spot가 남자에 있어서는 나타나지 않았고, spotc(39,000)의 군은 남자에 있어 여자보다 농도가 매우 낮았다. 임신한 여자에 있어 albumin이 임신 10주와 12주에 매우 감소하였고 그 이후에 다시 회복되었다. 그리고 spot f(70,000)는 임신 10주에 매우 감소 되었다가 그 이후에 증가되었다. 3. 각 군들의 아미노산을 분석한 결과 임신한 여자에 있어서 전반적으로 glutamic acid, lysine, aspartic acid, leucine 및 valine이 대체로 많은 양으로 나타났고, methionine, isoleucine 및 glycine이 대체로 적은 양을 가지는 것으로 나타났다. 전체 아미노산의 양은 임신중기에 현저히 증가하였다가 말기에는 중기에 비해 감소되었다. 이상의 결과를 볼 때 임신기간 중에는 모체의 혈청을 구성하는 단백질이 특정한기에 따라 증가하므로 임신한 여자의 혈청단백질을 조사함으로써 임신의 각기에 나타나는 독특한 단백질 양상 변화를 밝혀내는 동시에, 앞으로 좀더 연구함으로써 태아의 성을 단백질 패턴으로 명확히 구별해 낼 수 있을 것으로 생각된다.

For the comparative study of protein patterns of the sera of pregnant women, the protein in sera of normal male subjects, non-pregnant women, pregnant women and women delivered of children were analyzed by using the methods of SDS/polyacrylamide gel electrophoresis, two-dimensional electrophoresis and amino acid analysis. The results were as follows: 1. When the protein patterns of sera in normal male ranging from 10, 000 to 110, 000 daltons were compared to non-pregnant women by SDS/polyacrylamide gel electrohporesis, their protein patterns were same each other numerically but bands 3(22, 000 dalton) and 6(39, 000) were less in male than in non-pregnant women quantitatively. When the protein patterns in the pregnant women in which serum were collected two week intervals were compared with non-pregnant women, there was increased or decreased in several bands quantitavely. The protein patterns of sera in pregnant women were compared with those of non-pregnant women; band 3(22, 000) showed similar patterns each other until the 16th week but the quantity of protein was decreased continously from the 18th week to the third trimester of pregnancy. Contary, bands 4(24, 000), 9(69, 000), 10(70, 000), 12(80, 000), 14(86, 000), 15(91, 000) and 16(94, 000) were gradually increased in quantity from the begining of gestation, and band 7(51, 000) was increased until the 32th week of gestation only but somewhat decreased after this time. The quantities of bands 12(80, 000), 15(91, 000) and 16(94, 000) were relatively increased when the protein patterns of delivered women were compared with those of the third trimester of pregnancy. Women who were dilivered female children showed more increase in bands 4(24, 000), 7(51, 000) and 10(70, 000)than one who were delivered male chilren. 2. When the protein patterns of sera in normal males were compared with those of nonpregnant women by two-dimensional electrophoresis, three spots of spot a group were not appeared in the males and the spot c group in the males was less than in the non-pregnant women. In the pregnant women, albumin was significantly decreased during the 10-12 week of gestation but recovered after these times. And spot f(70, 000) was decreased in the 10th week of gestation but increased from this time. 3. Glutamic acid, lysine, aspartic acid, leucine and valine in pregnant women were large in quantity while methionine, isoleucine and glycine were small in quantity by amino acid analysis. The total amino acids were increased remarkably in the second trimester of pregnancy but began to decrease in the third trimester of pregnancy. As mentioned, this present paper deat with that proteins which consist of maternal serum were increased with specific period in pregnancy and that the change of characteristic protein patterns were identified in the serum protein of each trimester in the pregnant women. And furthermore, the study should be preformed for the sex-identification of a fetus in the early pregnancy.