Quantitative Analysis by Derivative Spectrophotometry (I) -Simulaneous quantitation of pyridoxine.HCI and nicotinamide in mixture by ultraviolet derivative spectrophotometry-

미분 분광 광도법에 의한 정량분석법 (제1보) -염산 피리독신과 니코틴아미이드 혼합물의 자외부에서의 분리정량-

Authors developed the computer application program (language: APPLE SOFT BASIC) for derivative spectrophotometry. By means of this program, derivative of spectral absorbance with respect to wavelength is recorded versus wavelength. To try this program in connection with spectrophotometer system, the authors have done the simultaneous quantitation of pyridoxine center dot HCl and nicotinamide in the mixture, and the result was compared with that of absorbance method.