계면활성제 첨가배양에 따른 Rhizopus oryzae의 $\alpha$-Amylase와 Phosphatase분비촉진

Enhancement by Surfactant on Release of $\alpha$-Amylase and Phosphatase in Submerged Culture of Rhizopus oryzae

Rhizopus oryzae를 액체배양하는 동안, $\alpha$-amylase, acid phosphatase 그리고 alkaline phosphatase와 같은 분비효소의 분비능에 대한 계면활성 제의 촉진효과를 조사하였다. 그 결과 집락의 형태적인 변화를 볼 수 있었는바, 0.18mM SDS 처리구에서는 작은 pelletal형, 0. 48mM DOC 처리구에서는 pulpy형, 계면활성제를 처리하지 않은 시험구에서는 사상형의 집 락을 나타내었다. SDS를 첨가함으로써 $\alpha$-amylase는 9배, acid phosphatase는 25배의 활성도 증가가 유도되었다. DOC첨가 시험구에서 alkaline phosphatase 활성도는 배양액에서 11배 증가가 나타났을 뿐 아니라 세포질에서도 역시 상승하였다.

Enhancement of surfactant on release of secretory enzyme, such as $\alpha$-amylase, acid phosphatase and alkaline phosphatase, was investigated during submerged culture of Rhizopus oryzae. Morphological changes of colony was occured; small pelletal form in 0.18mM of sodium dodecyl sulfate, pulpy form in 0.48mM of sodium deoxycholate, and filamentous form in absence of surfactant. It. Supplement of sodium dodecyl sulfate induced 9 times increasing activity of $\alpha$-amylase and that of acid phosphatase 25 times in cultural fluids. Alkaline phosphatase was increased 11 times in cultural fluid and also stimulated in cytoplasm with supplement of sodium deoxycholate.