Isolation and Bacteriological Characteristics of Spiral form Bacteria from Patient with Epidemic Pulmonary Hemorrhagic Fever

유행성 출혈형 폐염양 질환의 병원균 분리와 세균학적 특성

We have previously isolated seven strains of spiral form bacteria, which have shown several similar characteristics to Leptospira from patient with epidemic pulmonary hemorrhagic fever and matural paddy water. Further studies have performed to characterize them. All of the isolated bacteria were aerobic, gram negative and spiral form and their growth were completely inhibited at temperature below $13^{\circ}C\;and\;50^{\circ}C$. In sensitivity test to drugs, the bacteria were resistant to the bile salts, 5-Fu and Amphotericine B, but sensitive to the 8-Azaguanine. The isolated bacteria as well as 5 serotypes of Leptospira interrogans reacted the patient sera and it was also shown that antisera from rabbit immunized with the isolated bacteria reacted with 5 serotypes of Leptospira interrogans. These results suggest that the isolated bacteria may belong to the genus Leptospira.

유행성 출혈형 폐염양 질환의 환자 및 논물에서 분리한 병원체의 세균학적 특성을 조사한바, 형태적 및 물리생화학적 특성이 L.inteerogans와 동일하였으며 또한 형태학적 검사에서도 분류균과 L. interrogans는 항원적으로 같은 반응을 보였다.