Dynamic Studies on the Process of the Biological Denitrification 1. Variation of Bacterial Flora in the Waste Water Treatment of Fish Meat Paste Plant

생물학적 탈질공정에 관한 동력학적 연구 1. 연제품공장 폐수처리시의 세균Flora의 변동

This study was attempted to investigate variation of the bacterial flora in waste water treatment of fish meat paste plant by batch and continuous culture. The results of the experiment are as follows : 1. The removal rate of BOD in waste water treatment by activated sludge of continuous culture was above $90\%$. 2. In the process of nitric acidification of protein waste water, $NH_4-N\;and\;NO_2-N$ increased untill the lapse of 48 hours from culture, but $NO_3-N$ showed little change. 3. In activated sludge obtained from acclimation by batch culture for 10 days, bacteria good in capacity of nitric acidification were not appeared. 4. Among 120 strains of isolated bacteria, the most predominantly appeared bacterial flora were Enterobacteriaceae ($28\%$) and Pseudomonas spp. ($25\%$), In the latter term of aeration during which ammonia originates in abundance, Pseudomonas spp. was decreased but Enterobarteriaceae was increased. 5. Fifty percent of the isolated strains were able to grow in $0\%,\;3\%$ NaCl and $75\%$ artificial sea water, Therefore, it is suggested that sea water can be used as dilution water instead of tap water during the treatment of waste water.

단백질 폐수처리시의 세균학적 변동을 조사하기위해 연제품 가공공장 폐수중 기계적인 세정수를 시료로 하여 회분 및 연속배양을 행하여 얻은 결과는 다음과 같다. 1. 활성스럿지에 의한 연제품 폐수처리에서의 BOD및 COD 제거율은 모두 $90\%$이상으로 양호한 결과를 얻었다. 2. 단백질폐수의 질산화과정에서 $NH_4-N$와 $NO_2-N$은 48시간까지 계속 증가하는 경향을 나타냈으나 $NO_3-N$는 별 변동이 없었다. 3. 원료폐수중의 세균을 회분배양에서 10 일간 순양해서 얻은 활성스럿지 가운데는 질산화능이 우수한 균은 나타나지 않았다. 4. 분리균 120균주 가운데서 Enterobacteriaceae가 $25\%$, Pseduomonas가 $23\%$로 이 이속이 우점종이었고 암모니아가 다량생산되는 폭기후기에는 Pseudomonas는 감소하는 경 향을 보였고 Enterobacteriaceae와 Aeromonas가 출현율이 높았다. 5. 염분요구성은 120 세주중 $50\%$가 $0\%,\;3\%$ NaCl, $75\%$ ASW의 식염농도하에서 생육가능한 균이었음으로 농후한 단백질폐수의 희석수로 해수를 이용할 수 있다는 것을 알 수 있었다.