만손주혈흡충 충란항원 연속주입에 의한 면역학적 반응

Immunological Responses by Soluble Egg Antigen of Schistosomu munsoni in Mice

만손주혈흠충 충란의 soluble eff antigen (SEA)을 mini-pump를 이용하여 마우스, 복강내에 지속적으로 주입시키면서 말초혈액내의 호산구수 변동, delayed hypersensitivity인 ear swelling test, 간조직병변, 효소 표식면역법을 이용한 혈청내 항체가를 경시적으로 관찰한 결과 다음 성적을 얻었다. 1. 말초혈액 호산구수는 SEA투여후 2~3주후에 최고에 달하였고 그후 서서히 감소하였다. 2. Ear swelling test 실시에서 SEA투여 2주후에 종창이 최고에 달하고 그후 서서히 감소하였다. 3. 간조직병변은 SEA투여후 4주에 육아종형성을 관찰하였고 그후 조직손상이 복귀하였다. 4. 현청에서의 항체가는 SEA투여후 4주후에 양성대조군보다 높았으며 시간이 경과함에 따라 항채가가 증가하였다.

This experiment shows cellular and humoral immune responses induced by soluble egg antigen of Schistosoma manscni, that is, change of the number of peripheral blood eosinophil, delayed hypersensitivity measured by the degree of ear swelling, granulomatous change of liver tissue and elevation of serum antibody titer by ELISA. SEA was given continuously by the insertion of a minipump into peritoneal cavity of mouse. In control group, same pump with HGG was inserted. New pump was eachansed once In two weeks and followed the result until 9 weeks after mini-pump insertion. 1. Highest peripheral blood eosinophil level was recorded at 2∼3 weeks after SEA punp insertion, 2. MaRirnum ear swelling was observed at 2 weeks arid then decreased gradually. 3. In liver tissue, several granulomas without egg were formed at 4 weeks. 4. Serum antibody titer was eleyated from 4 weeks after SEA pump insertion.