The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Studies on Constituents of the Higher Fungi of Korea(XLI) -An Antitumor Fraction from the Culture Filtrate of Lentinus edodes DMC7-

한국산(韓國産) 고등(高等) 균류(菌類)의 성분(成分) 연구(硏究)(제41보)(第41報) -Lentinus edodes DMC7 균주(菌株)의 배양(培養) 여액(濾液)의 항암(抗癌) 성분(成分)-

  • Chung, Kyeong-Soo (Laboratory of Microbial Chemistry, Department of Pharmacy, College of Sciense Chungnam National University) ;
  • Choi, Eung-Chil (Department of Microbial Chemistry, College of Pharmacy Seoul National University) ;
  • Kim, Byong-Kak (Department of Microbial Chemistry, College of Pharmacy Seoul National University)
  • 정경수 (충남대학교 이과대학 약학과 미생물약품화학교실) ;
  • 최응칠 (서울대학교 약학대학 미생물약품화학교실) ;
  • 김병각 (서울대학교 약학대학 미생물약품화학교실)
  • Published : 1984.12.30
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Abstract

To find antitumor constituents in Korean basidiomycetes, the mycelia of Lentinus edodes DMC7, which had shown a good mycelial growth in shakeflasks, were cultured at $27^{\circ}C$ on an orbital shaking incubator at 180 rev/min for 12 days. The medium was composed of glucose (50g/l), yeast extract (9g/l), peptone (9g/l), and seven inorganic salts. A water soluble macromolecular fraction, LF-3, was obtained from the culure filtrate by fractionation with ethanol and dialysis using a Visking tube. When LF-3 was administered i.p. at 50mg/kg/day once daily for 10 consecutive days to female ICR mice which were implanted s.c. with sarcoma 180 $(10^6\;cells/mouse)$, it exerted a highly significant antitumor activity, with the tumor inhibition ratio of 53. 1%.

한국산 담자균류로부터 새로운 항종양 성분을 개발하기 위하여 표고의 일개 균주인 Lentinus edodes DMC7을 인공배지에서 진탕배양하여 그 균사 배양물을 얻었다. 그중 배양 여액으로부터 수용성 고분자 분획을 분리하여 LF-3라고 명명하였다. LF-3는 ICR 마우스에 이식된 Sarcoma 180세포에 대하여 항암작용을 나타내었으며 그 저지율은 53. 1%였다.

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