The Korean Journal of Mycology (한국균학회지)
- Volume 12 Issue 1
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- Pages.9-14
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- 1984
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- 0253-651X(pISSN)
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- 2383-5249(eISSN)
Some Factors Affecting the Protoplast Release from Pleurotus ostreatus
느타리버섯 균사(菌絲)의 원형질체(原形質體) 나출(裸出)에 관한 연구(硏究)
- Byun, Myung-Ok (Institute of Agricultural Sciences, Office of Rural Development) ;
- Go, Seung-Joo (Institute of Agricultural Sciences, Office of Rural Development) ;
- Park, Yong-Hwan (Institute of Agricultural Sciences, Office of Rural Development) ;
- Shin, Gwan-Chull (Department of Agricultural Biology, College of Agriculture, Chung Nam National University)
- Published : 1984.04.30
Abstract
Some factors affecting the protoplast release from mycelia of Plurotus ostreatus using commercial lytic enzymes were investigated. The highest yields of the protoplast were obtained from four days old mycelia grown in mushroom complete medium. The solution of 0.8M
느타리 버섯의 세포(細胞) 융합(融合)에 의(依)한 신품종(新品種)을 육성(育成)하기 위(爲)한 첫단계로 균사(菌絲)의 원형질나출(原形質裸出)을 위(爲)한 일련의 실험(實驗)을실시(實施)한 결과(結果) 다음과 같은 결론(結論)을 얻었다. 느타리 버섯 균사(菌絲)의 원형질체(原形質體)는 균사체(菌絲體)를 버섯 완전배지(完全培地)에 4일간(日間) 배양(培養)하였을 때 나출수(裸出數)가 가장 많았으며 삼투압 조절제(調節劑)는