• The Korean Journal of Mycology
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• v.12 no.1
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• pp.9-14
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• 1984

Some factors affecting the protoplast release from mycelia of Plurotus ostreatus using commercial lytic enzymes were investigated. The highest yields of the protoplast were obtained from four days old mycelia grown in mushroom complete medium. The solution of 0.8M $MgSO_4$, or KCI showed good results as the osmotic stabilizer for releasing the protoplast. Novozym 234 was the most effective among commercials tested. The concentration of the enzyme and pH of the enzyme solution were optimal at 15mg/ml and $5.5{\sim}6.0$ for the protoplast release, respectively. Mycelial digestion was optimal at about $28^{\circ}C$ and was better in the reciprocal shaking bath (75 oscillations/min) than the stationary culture.