Effect of 2,4-Dichlorophenoxyacetic Acid and Kinetin on Peroxidase Isoenzymes in Ginseng(Panax ginseng C. A. Meyer) Callus Cultures

고려인삼(Panax ginseng C.A.Meyer) 조직배양에서 2.4-dichloro phenoxyacetic acid와 kinetin첨가가 Isoperoxidase 변이에 미치는 영향

This study was undertaken to investigate the influence of kinetin and 2, 4-dichlorophenoxyacetic acid on the rate of growth, the contents of RNA, DNA, and protein. And also the effect of plant growth regulator on isoperoxidases in callus derived from root (root-callus) and petiole (petiolecallus) was investigated. The rate of growth in petiole-callus was higher than the rootcallus at 0.1 mg/l kinetin and 1mgfl 2,4-D. At 1mgll kinetic, the rate of growth increased, but at high concentration the rate of growth decreased fast. The contents of RNA, DNA and protein also increased, but it did not coincide with the increase of the growth rate of callus. The isoperoxidases of callus grown at various amounts of 2,4-D and kinetic occurred in an almost fashion, but those of root-callus appeared different from those of petiole-callus.