Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 10 Issue 4
- /
- Pages.289-295
- /
- 1982
- /
- 1598-642X(pISSN)
- /
- 2234-7305(eISSN)
Elimination of R-Plasmid in Streptomyces bobili (YS-40) by Ethldium Bromide
Ethidium Bromide에 의한 Streptomyces bobili(YS-40)의 R-Plasmid 제거
Abstract
Streptomyces bobili (YS-40) isolated from soil was tested that it had drug resistance against penicillin, cephalosporin series antibiotics and other antibiotics in the previous paper. The treatment of Streptomyces bobili, (YS-40) with ethidium bromide (EtBr), acriflavine and sodium dodecyl sulfate. (SDS) resulted in the elimination of R-plasmid from the host strain. Minimum growth inhibitory concentrations (MIC) of Hg, Ag, penicillin-G, ampicillin, chloramphenicol, oxytetracycline, streptomycin and kanamycin were found to be 15, 10, > 3, 000, > 100, > 1, 000, > 100, < 5 and < 5
Streptomyces bobili (YS-40) 의 Hg, Ag, penicillin-G, ampicillin, chloramphenicol, oxytetracycline, streptomycin, kanamycin에 대한 생육최소저해농도는 각각 15, 10, >3,000, >100, >1,000. >100. <5, <5
Keywords