Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 10 Issue 4
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- Pages.283-288
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- 1982
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Trypsin Inhibitor from Streptomyces sp. (Part 2) Biological Activities or the Inhibitor
Streptomyces 속 균주가 생성하는 Trypsin Inhibitor (제2보) 저해물질의 생물학적 작용상
- Yi, Dong-Heui (Department of Agricultural Chemistry, College of Agriculture, Kyungpook National University) ;
- Seu, Jung-Hwn (Department of Agricultural Chemistry, College of Agriculture, Kyungpook National University)
- Published : 1982.12.01
Abstract
Trypsin inhibitor produced by Streptomyces sp. was investigated its reactive characteristics against trypsin. The mode of inhibition against trypsin was mixed type of non-competitive and competitive with casein, and enzyme-inhibitor complex was formed rapidly. The inhibitory activity was increased by the addition of isoleucine and depressed by silver, mercuric or cupric ion. And when egg albumin or hemoglobin was used as substrate for trypsin, the inhibition ratio was changed. The inhibitor inhibited coagulation of blood of bovine.
Streptomyces속 균이 생산하는 trypsin inhibitor의 trypsin에 대한 반응성을 조사해 본 결과 본 저해물질은 crystalline trypsin (20.000 unit, hog pancreas)에 대하여 1/8량에서 약 50%의 저해률을 나타내었으며 trypsin에 대한 저해양상은 mixed noncompetitive-competitive inhibition type이었으며 enzyme-inhibitor complex를 빨리 형성하는데 반응액중 isoleucine이 공존하면 활성이 증가되였으며 Ag
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