Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 9 Issue 3
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- Pages.165-171
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- 1981
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Kinetic Study on the Immobilized Penicillin Amidase in a Differential Column Reactor
Differential column reactor에 있어서 고정화페니실린 아미다제의 반응속도론에 관한 연구
- Park, Jong-Moon (Department of Chemical Technology, Seoul National University) ;
- Park, Cha-Yong (Department of Chemical Technology, Seoul National University) ;
- Seong, Baik-Lin (Biotechnology Research Department, Korea Institute of Science and Technology) ;
- Han, Moon-Hi (Biotechnology Research Department, Korea Institute of Science and Technology)
- Published : 1981.09.01
Abstract
The penicillin amidase from Escherichia coli (ATCC 9637) was immobilized by entrappment in gelatin and DEAE-cellulose mixture cross-linked with glutaraldehyde, and the kinetics in a differential column reactor was studied. The optimal operating condition of a differential reactor was reasonably met when the enzyme loading was 1g, and 30 mM substrate solution in 0.1 M phosphate buffer (pH 8.0) was fed at flow rate 4
E. coil ATCC 9637의 균체를 젤라틴과 DEAE-cellulose의 혼합 성형 후 글루트알데히드 가교법으로 제조론 고정화 penicillin amidase의 differential column reactor에서의 반응속도를 논의하였다. 이러한 반응조의 최적 조작조건은 효소충진량 1g, 기질농도30mM(0.1M 인산완충액, pH8.0), 유출속도 4
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