Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 9 Issue 3
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- Pages.159-164
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- 1981
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Studies on Microbial Penicillin Amidase (Part 5) Application of Reinforced Calcium-Alginate Gel Entrappment Method for Immobilization of Penicillin Amidase from Bacillus megaterium
미생물 페니실린 아미다제에 관한 연구 (제 5보) Bacillus megaterium 페니실린 아미다제의 새로운 고정화 방법
- Son, Hyeung-Jin (Biotechnology Research Department, Korea Institute of Science and Technology) ;
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Seong, Baik-Lin
(Biotechnology Research Department, Korea Institute of Science and Technology) ;
- Mheen, Tae-Ick (Biotechnology Research Department, Korea Institute of Science and Technology) ;
- Han, Moon-Hi (Biotechnology Research Department, Korea Institute of Science and Technology)
- Published : 1981.09.01
Abstract
Reinforced Calcium-alginate gel entrappment method for enzyme immobilization is described with an example of penicillin amidase from Bacillus megaterium KFCC 10029, a partially constitutive mutant of B. megaterium ATCC 14945. Penicillin amidase recovered from the fermentation broth by adsorption on celite is mixed with alginate and gelatin solution, and cast into a pellet or noodle form by coagulation in calcium salt solution followed by crosslinking with glutaraldehyde. Optimum pH and temperature of the immobilized enzyme preparation were 8.0 and 6
부분적 항상성변이주인 Bacillus megaterium (KFCC 10029)가 생산하는 페니실린 아미다제를 예로하여 강화된
Keywords