냉습적(冷濕積)에 따른 은행나무종자내(種子內) 생장조정물질(生長調整物質)의 변화(變化)

Alteration of Endogenous Growth Substances in Cold-moist Stratified Seeds of Ginkgo biloba L.

휴면기간(休眠期間)이 긴 은행나무 종자(種子)의 냉습적처리(冷濕積處理)에 따른 생장촉진물질(生長促進物質)과 생장억제물질(生長抑制物質)의 증감(增減)을 구명(究明)하기 위하여 본(本) 연구(硏究)를 수행(遂行)하고 얻어진 결과(結果)를 요약(要約)하면 다음과 같다. 1. 무처리종자(無處理種子)는 배(胚), 배유(胚乳), 종피(種皮)의 각추출물(各抽出物)에서 생장억제물질(生長抑制物質)을 볼 수 있었으며, 특(特)히 종피(種皮)에서 가장 현저(顯著)하게 나타났다. 2. 냉습적처리종자(冷濕積處理種子)의 배(胚)와 종피(種皮)에서 생장억제물질(生長抑制物質)의 현저(顯著)한 감소(減少)가 있었으나, 생장촉진물질(生長促進物質)은 배부분(胚部分)에서 증가(增加)가 약간 나타났다. 3. 이상(以上)의 결과(結果)에서 은행나무 종자(種子)의 휴면(休眠)에는 종피(種皮), 배(胚), 배유(胚乳)의 각부분(各部分)이 생장억제물질(生長抑制物質)이 존재(存在)하며 발아(發芽)에 관계하는 것으로 추측(推測)된다. 4. 은행나무 종자(種子)와 발아촉진(發芽促進)과의 관계는 생장촉진물질(生長促進物質)의 증가(增加)보다는, 배(胚)와 종피(種皮)의 생장억제물질(生長抑制物質)의 감소(減少)에 영향된다고 추측(推測)된다. 5. 생장촉진물질(生長促進物質)인 Gibberellin은 자외선형광검사등검정(紫外線螢光檢査燈檢定)에서 Rf 0.26, 에서 나타났고, 생장억제물질(生長抑制物質)인 Abscisic acid는 Rf 0.62, Rf 0.70, Rf 0.78에서 각각(各各) purple, gray, blue의 형광색(螢光色)으로 나타났다.

This study has intended to disclose the change of some chemical compositions of Ginkgo seeds which were acquired the treatment of cold-moist-stratification after collection. As check sample, the room-stored seeds were used. With the reasons that when the seeds not stratified were sown the delay of field germination has usually been resulted, the effectiveness of stratificaation in respect to alteration of chemical composition is to be investigated. The increase and decrease of growth promoting and inhibiting substances were investigated by means of chromatography method followed by rice seedling test or wheat coleoptile straight-growth test. The results obtained are summarized as follows; 1. In the untreated seeds, the zone of growth inhibitors on paper chromatograph were observed without regard to the tissue differences, embryo, endosperm and seedcoat. 2. Due to stratification, the amount of inhibitor has decreased in the embryo and seed coat, but growth promoters was decreased as compared with the check materials 3. The indications of results appear that each portion of the embryo, endosperm, and seedcoats of Ginkgo biloba L. contains the growth in hibitor taking part in germination dormancy. 4. It was presumed that hastening germination was influenced by decreasing of inhibitors in the embryo and seed coats rather than by increasing of promoters. 5. Gibberellin was detected at Rf 0.26 under the UV-lamp and the abscisic acid was detected at Rf 0.62, Rf 0.70, and Rf 0.78 and showed purple, gray, blue fluorescence respectively under the UV-lamp.