염화(鹽化)트리페닐테트라졸륨에 의(依)한 종자활력검정(種子活力檢定)에 관(關)한 연구(硏究)

Seed Variability Test by 2,3,5-Triphenyltetrazolium Chloride

임목종자(林木種子)의 활력검정(活力檢定)에 2,3,5-triphenyltetrazolium chloride 0.25-0.5%의 수용액(水溶液)으로 처리(處理)하여 얻은 결과(結果)는 X-ray 촬영법과 동일(同一)한 효과(効果)를 얻어 본(本) 시약(試藥)의 음용이 값싸고 우수한 종자활력검정(種子活力檢定) 방법(方法)임이 판명되었다.

To elucidate the dependability of seed variability determination by tetrazolium, 15 tree species were checked. For the comparison of potential germinability for these tree seeds, x-ray photography and routine germination test in a incubator were implemented. Though there were considerable differences of seed variability in percentage between germination test and tetrazolium test, the judgement by tetrazolium test and x-ray test gave similar results. These findings indicated that tetrazolium test is a reliable method if treated and inferred properly. Solution of 0.25 percent tetrazolium could give a complete colour reaction as well as 0.50 percent solution.